Date of Degree
Master of Science (MS)
Osajin and pomiferin, prenylated isoflavones extracted from the fruit of the osage orange tree (Maclura pomifera) have been reported as antioxidant compounds. The purpose of this study is to analyze the metabolization of osajin and pomiferin by the fungi Cunninghamella elegans (ATCC 9245) and supporting strains Umbelopsis ramanniana and Aspergillus fumigatus fresnius.
HPLC analysis of the extracts showed new, more polar compounds were formed, evidenced by peaks at lower retention times for each strain of fungi. To further investigate the metabolites produced, HPLC-guided chromatographic purification will be performed, and the pure metabolites will be analyzed through nuclear magnetic resonance and mass spectrometry.
Luis, Stephen, "Biotransformation of Natural Antioxidants Osajin and Pomiferin by Cunninghamella Elegans (ATCC® 9245TM)" (2019). Theses & Dissertations. 359.